Journal: Oncology Letters

Article Title: Pathologic evaluation of tumor-associated macrophage density and vessel inflammation in invasive breast carcinomas

doi: 10.3892/ol.2017.6466

Figure Lengend Snippet: Association between clinicopathologic parameters, and CD68 and E-selectin expression in procedure-naïve invasive breast carcinoma tissues.

Article Snippet: Sections were incubated with mouse anti-E-selectin monoclonal antibody at 1:100 (cat. no. MO20039; Neuromics, Inc., Minneapolis, MN, USA) overnight at room temperature.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Pathologic evaluation of tumor-associated macrophage density and vessel inflammation in invasive breast carcinomas

doi: 10.3892/ol.2017.6466

Figure Lengend Snippet: TAM and E-selectin expression levels in breast tumor tissues. (A-C) Single immunohistochemical staining of CD68 + TAMs for analysis of the distribution at various areas of the invasive breast carcinoma tissues (red). (D-F) Differential E-selectin expression in vessel surface in breast carcinomas (brown). The images are representative at a final magnification of ×200. Scale bar indicates 100 µm. V, vessels; A, adipocytes; CD68, cluster of differentiation 68.

Article Snippet: Sections were incubated with mouse anti-E-selectin monoclonal antibody at 1:100 (cat. no. MO20039; Neuromics, Inc., Minneapolis, MN, USA) overnight at room temperature.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Oncology Letters

Article Title: Pathologic evaluation of tumor-associated macrophage density and vessel inflammation in invasive breast carcinomas

doi: 10.3892/ol.2017.6466

Figure Lengend Snippet: Double immunohistochemistry of TAM and E-selectin. (A and B) Spatial association of CD68 + TAM and E-selectin in the tumor stroma of invasive breast carcinoma tissues. (C) Absence of E-selectin expressing vessels in necrotic area. (D) Presence of CD68 + TAM and E-selectin in stroma of non-carcinoma area of invasive carcinoma tissue. Brown indicates E-selectin and red indicates TAM. The images are representative at a final magnification of ×200. Scale bar indicates 100 µm. V, vessels; C, cancer cells; N, necrotic area; CD68, cluster of differentiation 68.

Article Snippet: Sections were incubated with mouse anti-E-selectin monoclonal antibody at 1:100 (cat. no. MO20039; Neuromics, Inc., Minneapolis, MN, USA) overnight at room temperature.

Techniques: Immunohistochemistry, Expressing

Journal: Oncology Letters

Article Title: Pathologic evaluation of tumor-associated macrophage density and vessel inflammation in invasive breast carcinomas

doi: 10.3892/ol.2017.6466

Figure Lengend Snippet: Association between CD68 + TAMs, and E-selectin expressing vessels and tumor inflammation in procedure-naïve invasive breast carcinoma tissues.

Article Snippet: Sections were incubated with mouse anti-E-selectin monoclonal antibody at 1:100 (cat. no. MO20039; Neuromics, Inc., Minneapolis, MN, USA) overnight at room temperature.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Pathologic evaluation of tumor-associated macrophage density and vessel inflammation in invasive breast carcinomas

doi: 10.3892/ol.2017.6466

Figure Lengend Snippet: Overall survival by prevalence of (A) tumor inflammation, (B) TAM and (C) E-selectin. Kaplan-Meier survival curves according to tumor inflammation, CD68 + TAMs and E-selectin + vessels in 53 invasive patients with breast cancer. TAM, tumor-associated macrophage; CD68, cluster of differentiation 68.

Article Snippet: Sections were incubated with mouse anti-E-selectin monoclonal antibody at 1:100 (cat. no. MO20039; Neuromics, Inc., Minneapolis, MN, USA) overnight at room temperature.

Techniques:

Journal: Oncology Letters

Article Title: Pathologic evaluation of tumor-associated macrophage density and vessel inflammation in invasive breast carcinomas

doi: 10.3892/ol.2017.6466

Figure Lengend Snippet: Multivariable Cox proportional hazard regression analysis of overall survival.

Article Snippet: Sections were incubated with mouse anti-E-selectin monoclonal antibody at 1:100 (cat. no. MO20039; Neuromics, Inc., Minneapolis, MN, USA) overnight at room temperature.

Techniques: Expressing